| Well , first the sample needs to be fresh. Secondly, you need to add that drop of water as I suggested. Third, you need to work fairly quickly as time, slide cover pressure and most of all- the light warm and kill these individuals pretty quickly.
The best way to do this is to start with low power ( you should be able to see the entire field like an aerial photo. Then go to a higher power, refocus and start at the upper left corner and slowly move to the right. Like reading a sentence in a book, when you get to the end of the slide, drop your view down to the 'next line' and now move right to left ( as if you were now reading the next sentence in the book backwards. Once you get to the of the cover slip, repeat, by dropping down and 'reading' left to right again. This way you will scan the entire slide missing very little.
the only way this will NOt work is if your lighting is too high and the light actually passes right thru the thin walled parasite, making it almost invisible. JR |