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Culture and WAIT for results? We don't even do that with humans 99% of the time. By the time you WAIT for that to happen, things could get too far along.
To decide whose advice to follow, I would recheck the fish in 24 hrs. If "ulcer" appears worse, inject. If better, leave alone and observe carfully. If the same, make sure no raised scales around border and recheck next day.
Over the years, I've injected amikacin in many koi, almost always it just takes one injection and the ulcer appears better in 24hrs - if so, I do not reinject, just let fish continue on its own from this "jump-start" of the healing process. Amikacin is a STRONG antibiotic, and too many doses in succession (three +) is too hard on the fish, in my opinion. Follow correct dosage table carefully.
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