The best information always comes from reliable sources. I was instructed by Vicky in person, at the Koi Health Seminar at UGA that the best, and preferred, protocol for serology samples sent to her should be followed in this manner:
-Heat the Koi at 74 degrees for 2 weeks
-Take blood samples from live specimens
-Place samples in a serum-separator tube
-Place samples in centrifuge and spin 10-15 min.
(depending on mfg. instructions)
-immediately freeze samples
-overnight to her at UGA
I know my customers will feel safer when I have used the best possible protocol for testing, so I do not consider doing anything less...