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Ah, JR, I didn't use a smiley-thing, so my sarcasm came across as serious. Although there was a little seriousness in it. I have the impression that the very experienced can reach a high percentage of correct sexing decisions on about half the tosai they examine. The other half are indeterminate. But even with the group that they believe they know the sex, some turn out different. So, no, tosai cannot be sexed 100%. ...I have a male Kohaku that went through very experienced hands as a female when nisai. As sansai, it was definitely a male, although looking like a small female to the eye of most. "Guaranteed female" is only as good as the seller's ability and willingness to replace with an equivalent female.
Now, for bloodlines ... first, I think someone needs to define what they mean by a "bloodline". If they think there is any bloodline in koi that gives a purebred as in dog breeding, they need to think again. Even in the most refined Kohaku there are shiromuji and benigoi produced. The real question for the breeder is the success of oyagoi pairings in producing the most profitably marketable quantity of offspring. Sakai of Hiroshima succeeds due to the incomprehensible millions of fry produced each year. Out of 50 million fry, something good is likely to show up if you have the eye to recognize it. Mat McCann told me that a pairing that produced one of the best offspring he has obtained is not being repeated because there were so few marketable out of the match. A different match producing many marketable offspring is preferred, even if that match does not produce an individual koi at the level of the one-off. Perhaps a Sakai can use the resources of mud pond space and long afternoons of culling to produce a single marketable fish of special note, but not many fish farmers can take that risk. Can a person say there is a bloodline when over 90% of the offspring are worthless and get tossed by the side of the pond to feed the ants? ...It may be a bloodline in the sense that some genetic characteristics are more observable in the offspring of a certain match than in others, and maybe there are even certain traits that are prevalent. But, unlike dogs and racehorses, bloodlines in koi are more marketing than real. Is there any highly regarded breeder who does not regularly acquire new breeding stock from unrelated lines to try for improvement? A famous female may be touted as the parent fish, but check the males. They are often unrelated "bloodlines". ...the resulting fry can hardly be said to be of any "bloodline". Perhaps instead of saying a koi is "MagnaBeni Bloodline", it would be more accurate to say: "It took 8 gene pools and 3 generations to get this fine fish."
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