| I've always been a fan of "seeding the filter" with used media and water from a pre-existing pond. You did use water from your old pond, so assuming the rest of the water was well dechlorinated in the first place you did at least drop in a wee bit of active bio, but probably not enough to do you much good.
At these water temperatures the only real ammonia source will be the slime coat and minimal wastes from the Koi in the pond, so getting your bio to kick off will be painfully slow as already mentioned. The good news is that at low temperatures and moderate ph levels ammonia toxicity is nil, so your fish should be fine for now. They will produce precious little excrement in a virgin pond like this as there is no algae present for them to munch on over the winter.
If they've been surviving in a 1,000 gallon pond for 2 years, an 8,500 gal pond should feel like paradise. Just my opinion, but I would think it would be fine to move 1 per week until they are all moved. So long as ammonia tests remain in check that is. If the ammonia becomes a concern, the chloramex you would use for a regular water change should be sufficient to handle the ammonia as well without starving your filter for food.
You might consider using a heater in the biofilter. Warming the filter water a bit may improve your bio response even though the pond water is still cool.
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Larry Iles
Oklahoma
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