Hello nguyen4 . . .
Like I posted to dg in her thread, there are many, many threads here on K-B with detailed discussions on sexing koi.
You can always use the search function to find a couple of the most recent ones, and then ask followup questions. This is a complex issue and lots of study will be required to master it.
For starters, here's one on sexing koi:
http://www.koi-bito.com/forum/general-koi-forum/6495-future-koi.html
pd . . .
Checking the vent is a critical part of the labor intensive culling (sembetsu) process.
And since a female is worth roughly 3 times what a male is (a convenient rule of thumb for equal quality fish), failure to properly sex tosai at the 80%+ level while culling can mean the difference between the farm having a successful harvest and a losing one.