Welcome to the forum.

Strap on your thinking cap, you can learn a lot here.
Simple answer to your question, no. UV isn't latent. It will kill anything free floating in the water column (good or bad) that passes through it (given your flow rates are correct). However, the bacteria that takes care of your nitrification process will be on your filtration media and pond surfaces.
Could you tell us a bit more about your pond? I'm not one of them, but there are some amazingly experienced people on this board and the more info they have about your system, the better they can assist you. Things like :
design/shape of your pond (vertical sides/sloping sides, liner/concrete, etc)
pump flows
style of pump (submersible/external)
filter manufacturer and type or description of what ya built!
type of koi/number of koi you'd like to keep
Anyway, that's a start and have fun!
Grant