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Old 06-29-2005   #11 (permalink)
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I have been messing around with the "probiotics" used by shrimp-farmers. With the limited data I have for a low cost food, it seems to have reduced amount of (floating)feces. The amount of of feces increased when the probiotics was omitted. This is repeatable...at least twice.

Perhaps the microbes have more influence than we thought?
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Old 06-30-2005   #12 (permalink)
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REC: Welcome! And thanks for the input. But, why are you keeping your results secret? ... Many omnivorous fish consume feces that settle out of the water while scrounging around the bottom. I'd expect koi to re-consume quite a bit while grazing on algae on a pond bottom. Good or bad? Perhaps we do not want the bottom drains to be excessively efficient? LOL.
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Old 06-30-2005   #13 (permalink)
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No Secrets..

Hi Mike.. thanks for the welcome. I am not keeping it secret but would like for someone like Mark to try this so we can compare notes. Iused ShoKoi and would be interested in the results using another brand of koi food.

Your point about re-ingesting feces is a good one and indeed, I am sure koi will do exacly that. But consider what is left after the first digestion process... much of the same basic substance but reduced by the extraction of the minerals, nutrients, etc. Koi feces, unlike mammal, are not dramatically changed by digestion process. However, for those who like to buss their fish, you might want to think twice after reading this thread...

We do know that some parasites that affect the gut are transmitted by re-ingesting feces with the parasite intact. For whatever reason, Mother Nature designed this form of transmission.

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Old 06-30-2005   #14 (permalink)
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Hi REC and welcome! It seems to me after reading your post that a food packed with the nutrients a koi needs would indeed make less waste as the koi's digestive system would process that for use and leave less waste. Or if that is not the case then if the food just breaks down into tiny particles that are not trapped by the filter that would also be benificial as it would eventually either settle in a seperator or would be drained out during a water change. Either way it wouldn't be coated with mucus and be eaten by the aeromonas in the filter right? Or am I way off base?
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Old 06-30-2005   #15 (permalink)
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Rec,



My search is over! I finally found someone with the same level of passion about koi feces as myself.

To gauge the health of my koi I have installed a stainless screen (105 microns) in a settlement chamber. A daily check of filtered feces can communicate a great deal about the health of koi and is the best early warning system I know of.

My feed experiments are going to be centered on three main parameters: Feed composition, temperature, and gut bacteria colonies. I feel what a koi consumes could likely be the factor between a good koi and a great koi, with all other factors being the same. Those fast growing 2 year olds need 110% of their nutrients on demand and in time. Any deficit in nutrient or mineral intake (absorption) could conceivably alter the kois development irrevocably for the worse.

I’ve been successful in maintaining 16 bacteria cultures for probiotic use. There are more than 100 bacteria species that could be suited for this purpose. The problem I have encountered is replacing the resident bacteria colonies in the gut with the bacteria of choice. This is only preliminary, but I think the media carrying the new bacteria colonies need more contact time in the gut to establish themselves coupled with a higher density bacteria count. Might also use a flushing method to compliment the introduction method.



Well, I could on and on about this fascinating subject but I think I take it up on a new thread. Will post details and pictures on the lab within 3 weeks.



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Old 08-26-2005   #16 (permalink)
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Originally Posted by MikeM
I often come across comments that a food is "more digestible" as proved by the fact that there is "less waste". I do not understand the supposed connection.

A fish is largely composed of water. If you have ever had a fish jump out and dry out completely, you know that the dry weight of the fish was a very small percentage of the natural weight. Very little of any dry food is going to be absorbed by a fish through the digestive process. Virtually everything that is consumed will be expelled. So, how can there be a noticeable difference in waste volume?

Seems to me that there are two possibilities: (1) the food is so nutritious that less dry weight of it is fed [note: not less volume, but less dry weight...some foods are full of air], but I've not noticed people commenting that they feed less of particular foods on a weight basis; or, (2) the waste is just as voluminous, but not as easily observed.

Wheat germ is often identified as a highly digestible food. In mammals, wheat germ has a mild laxative effect. I expect the same is true of fish. During cool weather this can be very desirable, because we do not want food rotting in clogged-up intestines. However, the reason wheatgerm has a laxative effect is because it contains indigestible fiber, high oil content, and low starch levels (as well as high protein content). It results in less visible waste because the starches which bind waste into visible stools are very low. This does not necessarily mean the fish are getting more nutrient out of the food.

So, I see the photo on the Saki-Hikari label showing filter mats with less visible waste trapped and I wonder: If the waste is no longer getting captured in the matting, where is it? [If there is 50% less visible waste, are the koi gaining dry weight every meal equal to 50% of the weight of the food fed? ... Of course not. ] Wherever it is, is it better for the fish that the waste is not visibly trapped in the matting? Do I want waste that does not settle out in the vortex chamber? Or, do I want solid stools that I can see and that do settle out in the vortex without breaking apart?

I really do not know the answers. I have purposefully phrased my thoughts to create doubt about the usual "less visible waste means better digestibility" concept. That concept may be wrong, but the foods involved could still be the best for a different rationale.

Anyone aware of published scientific studies on digestibility in fish and absorption of nutrient from different food sources?
Digestibility & Waste



I had a friend do a little research for me, on this post.


I often come across comments that a food is "more digestible" as proved by the fact that there is "less waste". I do not understand the supposed connection.

A fish is largely composed of water. If you have ever had a fish jump out and dry out completely, you know that the dry weight of the fish was a very small percentage of the natural weight. Very little of any dry food is going to be absorbed by a fish through the digestive process. Virtually everything that is consumed will be expelled. So, how can there be a noticeable difference in waste volume?



NOTE: Actually your comment “very little of any dry food is going to be absorbed by a fish through the digestive process” is not and if you think about it logically cannot be correct. Just for your reference, the lab guys tell me 67% to 80% of the body weight of a fish is water, but that’s another subject. The most important factor in any koi diet is the balance of the formulation. This means there has to be a balance between the proteins, carbohydrates and fats that will allow the koi to properly utilize the nutrition. Of course, there also has to be certain vitamins and minerals that round out the diet. The objective of the diet producer is to develop a diet that will allow the fish to use the nutrition from the food for kinetic energy (metabolizing action) which allows the koi to remain active and maintaining their health. This conversion of the nutrition to usable energy has to take place or the fish would steadily lose body mass until they eventually died. Furthermore, through this action some of the nutrition (assuming the diet is a proper one of course) is absorbed to provide growth. The main by-products of this activity are carbohydrates and ammonia. So >from a clinical perspective you have the following action taking place:



Fish Food Weight = increased body weight + released CO2 + released slime coat + other released chemicals including NH3 + the dry weight of the feces



In this instances a high amount of the nutrition will result in CO2 and chemicals like NH3, but without a proper nutritional balance or source the first sign would be increased feces which might relate more to your point above. This is not a normal occurrence for a well-balanced and complete diet! Always remember, as the digestive properties of the food are reduced, (to save money or increase production output which saves money) less and less of the nutrition can be absorbed and thereby utilized by the fish. When this occurs it has to exit the fish as waste.



As another example, if you have diet A that is 80% efficient and diet B that is 40% efficient, you would have to feed twice as much of diet B to give the koi the same amount of nutritional input as diet A. This means that instead of 20% of the amount of diet A being excreted as unusable solids there would be almost 6 times the amount put out in the water. A significant and noticeable increase no doubt! Of course, this is an extreme example, but it gives you a very clear picture.

Seems to me that there are two possibilities: (1) the food is so nutritious that less dry weight of it is fed [note: not less volume, but less dry weight...some foods are full of air], but I've not noticed people commenting that they feed less of particular foods on a weight basis; or, (2) the waste is just as voluminous, but not as easily observed.



NOTE: Of course it stands to reason that the better the digestibility of the diet the less food you have to feed to accomplish the same growth as the alternative diet. Waste is waste and in most ponds it’s quite visible. If not, any filter maintenance would certainly provide a visible answer to this question. The fact of the matter is if you used the two diets above A & B and fed the same amount of both (dry weight) you would experience faster growth and less waste with diet A and more noticeable waste and less growth with diet B. The real question is do you want to put some of your fish through this drill to prove the point?



Wheat germ is often identified as a highly digestible food. In mammals, wheat germ has a mild laxative effect. I expect the same is true of fish. During cool weather this can be very desirable, because we do not want food rotting in clogged-up intestines. However, the reason wheatgerm has a laxative effect is because it contains indigestible fiber, high oil content, and low starch levels (as well as high protein content). It results in less visible waste because the starches which bind waste into visible stools are very low. This does not necessarily mean the fish are getting more nutrients out of the food.




NOTE: Technically your statement here is correct, wheat-germ is naturally high in dietary fiber, so if you were to feed only wheat-germ (outside of a well-balanced prepared diet) you could expect poor results at best. The key to our diet is utilizing the wheat-germ for its benefits along with a complete balance of other ingredients that help accelerate digestion while providing the koi with the nutrition they need to remain healthy in cooler water. It’s important to remember, one ingredient does not make a koi food!



So, I see the photo on the Saki-Hikari label showing filter mats with less visible waste trapped and I wonder: If the waste is no longer getting captured in the matting, where is it?




NOTE: With this diet we are experiencing three primary activities with the food that help to bring about this result. (1) Extremely digestive ingredients are utilized to help the koi convert the nutrition to kinetic energy and usable nutrition for growth. (2) The enzymatic action in the digestive tract is increased by the beneficial bacteria further allowing the koi to utilize the nutrients in the food. A by-product of this action is a heightened level of available vitamins which help improve metabolism (3) the action of the beneficial bacteria continues once the waste is excreted from the body of the fish and works to decompose the waste that remains. This is a short term process yet is quite successful at reducing overall waste volume.



[If there is 50% less visible waste, are the koi gaining dry weight every meal equal to 50% of the weight of the food fed? ... Of course not. ]



NOTE: Your comment above oversimplifies the whole process taking place once the food is fed. The waste reduction is a result of multiple activities taking place simultaneously, not just one. In the absence of the beneficial bacteria, less visible waste would have to mean more absorption of the nutrition which would result in increased levels of kinetic energy for the fish to utilize or a noticeable improvement in the growth.



Wherever it is, is it better for the fish that the waste is not visibly trapped in the matting? Do I want waste that does not settle out in the vortex chamber? Or, do I want solid stools that I can see and that do settle out in the vortex without breaking apart?



NOTE: I would think that what would be desirable for any pond keeper is waste that can more readily be handled by the bacterial action of your filter. More efficiency in this area will result in less maintenance, better water quality and potentially less health issues for the koi. It’s not a matter of now you see it now you don’t in this instance. If the beneficial bacteria work to decompose the waste, it is simply not there. If this action results in a finer waste that the bacterial process can break down, good things can result!



I really do not know the answers. I have purposefully phrased my thoughts to create doubt about the usual "less visible waste means better digestibility" concept. That concept may be wrong, but the foods involved could still be the best for a different rationale.




NOTE: I think it’s important to note here that Hikari is the only koi diet producer in the world that breeds koi. The results of any diet we bring to market have already passed the test of time in real-live conditions before our very eyes. We already know the diet’s impact on the fish in the short term and longer term before any consumer tries it on their pets. Furthermore, this first-hand insight coupled with an extremely modern research lab full of folks that know fish and can readily research the impact of nutritional ingredients, means we have a huge advantage over the competition. Add in our own production facilities that can guarantee the quality from start to finish and you have a clear picture why more koi breeders ask us to produce diets for them than all the other koi food makers combined. In the end, it’s not what we say any of our diets can do, it’s what the consumer sees them doing in their own situation and how the koi respond that matters most to us.
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Old 08-26-2005   #17 (permalink)
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Wow, eating there own .... That's not nice is it. For a general rule with quality digestible food (no corn) diet. Out of 100 units of feed fed to fish, typically about 10 units of feed are uneaten, wasted 10 units of solid and 30 units of liquid waste so 50% total wastes are produced. The remaining feed 25% is used for growth, another 25% is used for metabolism, heat, energy for life process. So 25 units for body weight and 25 units for energy to run the equipment.
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Old 08-26-2005   #18 (permalink)
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THANKS NANCY FOR THE TIME AND ENERGY YOU PUT INTO YOUR REPORT!

tHIS HAS BEEN A GREAT THREAD!
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Old 08-26-2005   #19 (permalink)
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Quote:
Originally Posted by dick benbow
THANKS NANCY FOR THE TIME AND ENERGY YOU PUT INTO YOUR REPORT!

tHIS HAS BEEN A GREAT THREAD!
Hi Dick
I just wanted to get the information to see if I could understand it better, and also to help others.
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Old 08-26-2005   #20 (permalink)
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Another country heard from--


koi are cold blooded and as such have a set point for maximum digestion- 68- 76 F. Note, this does not mean they can't eat out of this range but they absorb the nutrient less efficiently outside this range.
number two- no pun intended, is a function of how much is ingested, digested, absorbed and then assimilated. This is a coordinated process in which the koi must burn calories to gain calories- this is why temperature is so important for performance.
number three- koi are a total organism. Water conditions will dictate how well digested food is absorbed into the body. If the koi is 'off' white feces will be produced. Ironically, much of the excess food that is fed/overfed to a koi actually passes as partly digested organic and deteriorates water quality and causes less absorption in subsequent meals. It is a case of what is absorbed and assimilated -- not what is ingested. Less is often more.
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